Metabolic engineering for alternative pathways to capture CO2
in the yeast Pichia pastoris
in Vienna / Austria for 30 hours a week
That’s the project
Towards a more sustainable biotechnology, it is of great interest to create autotrophic or mixotrophic industrially relevant microorganisms that are able to incorporate CO2 to their central metabolism as this would help to mitigate atmospheric CO2. Recently, we have integrated the Calvin-Benson-Bessham (CBB) cycle into the yeast Pichia pastoris (Gassler et al. 2020, Nat. Biotechnol. 38:210-216). As a methylotrophic yeast, the autotrophic P. pastoris strain uses methanol for energy supply and can grow on CO2 as sole carbon source. In this PhD project, we aim to design new CO2 capturing pathways mixed with methanol uptake and explore them for the production of biochemicals.
By using metabolic network modelling and the state-of-the-art synthetic biology methods such as Golden Gate Assembly and CRISPR-Cas9, we aim to design thermodynamically feasible and energetically efficient pathways for mixed feed of methanol and CO2 and test the generated strains in both small scale and bioreactor cultivations.
Research will take place in the research group “Metabolic and Cell Engineering” at the Department of Biotechnology of the University of Natural Resources and Life Sciences, Vienna (BOKU)
That could be your future
You are working in an enjoyable work climate, have numerous possibilities for further education and personal development.
You are part of a challenging and helpful work environment full of team spirit and enthusiasm for your job. You expand your expertise and bridge your science to industry.
That’s important to us
Completed Master’s degree (or equivalent) in biotechnology, biochemical engineering, molecular biology, genetics, biochemistry, or similar study.
Background in biochemistry, synthetic biology, biotechnology, or related disciplines.
Practical experience in molecular biology and/or metabolic modelling requested, and scientific interest in bioinformatics applications and bioreactor cultivation desired.
Excellent skills in spoken and written English, an organized approach with strong attention to detail and good communication skills with ability to work well in teams.
That’s what you should know
Minimum yearly gross payment of EUR 30,870 (PhD 30 h/week).
Planned start: November 2020
Find out more about why acib is the right choice for you: www.acib.at/career
And now it’s your turn! Give us an idea of who you are.
Apply here: https://mycareer.acib.at I Ref.no: 13_2020
Upload a detailed CV and publication list, a motivation letter, copies of university degree documents, contact information (phone number and email) and contact details of up to 3 academic referees
Application deadline: 31st of August 2020
Of course, we welcome applicants of all sexes (m/f/x), backgrounds and ages.
acib (The Austrian Centre of Industrial Biotechnology) is one of the leading research centres in the field of industrial biotechnology.
acib is dedicated joint research at the industrial-academic interface.
Universities and industry partners are working together within the acib research programme.